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Genetic mapping of a putative tumor suppressor locus that influences tumorigenesis and metastasis in mice
Author(s) -
Ayanga Bernard,
Price Roger,
Gu Xiangjun,
Lozano Guillermina,
Evans Susan C.
Publication year - 2006
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.20328
Subject(s) - loss of heterozygosity , biology , locus (genetics) , carcinogenesis , suppressor , allele , tumor suppressor gene , gene , null allele , genetics , cancer research , microbiology and biotechnology
The tumor suppressor TP53 is mutated in ∼70% of Li‐Fraumeni syndrome (LFS) families; however, other genes may lead to the predisposition to tumors in other families. We developed a mouse model to search for other tumor suppressors that may be involved in the syndrome. Inbred CE/J mice, which succumb to multiple types of tumors similar to those found in LFS, were crossed with the Trp53 ‐null 129‐ Trp53 tm1Tyj mouse. We monitored the tumor onset and type and found a significant earlier tumor onset in the CE/J:129‐ Trp53 tm1Tyj mice compared with 129‐ Trp53 tm1Tyj mice with a Trp53 ‐null allele. Additionally, in CE/J:129‐ Trp53 tm1Tyj‐ Trp53 +/− mice, the tumors metastasize, which does not occur in other strains of mice. Using simple‐sequence length polymorphism analysis for loss of heterozygosity in tumors, we identified a putative tumor suppressor locus within 1 cM on mouse chromosome 11, which encompasses 12 mapped genes. © 2006 Wiley‐Liss, Inc.