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FISH studies identify the i(20q−) anomaly as a der(20)del(20)(q11q13)idic(20)(p11)
Author(s) -
Li Tianyu,
Xue Yongquan,
Wu Yafang,
Pan Jinlan
Publication year - 2006
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.20313
Subject(s) - dicentric chromosome , biology , centromere , fluorescence in situ hybridization , fish <actinopterygii> , locus (genetics) , chromosome , chromosome 20 , chromomycin a3 , genetics , cytogenetics , microbiology and biotechnology , karyotype , gene , fishery
Fluorescence in situ hybridization (FISH) analyses were performed on six of seven patients who had been reported in 2004 to have an i(20q−) anomaly expressed as ider(20)(q10)del(20)(q11q13). The i(20q−) was investigated with a series of probes: a centromere‐specific probe for chromosome 20, two paint probes for 20p and 20q, and a panel of locus‐specific probes prepared from BAC/PAC clones mapped to 20p. The results showed that: (1) i(20q−) was a dicentric chromosome; (2) both of its arms comprised a deleted 20q and a small part of 20p near the centromere of chromosome 20; and (3) the breakpoints and reunion sites of i(20q−) differed, residing in the region 20p11.21–20p11.22 delineated by BAC/PAC clones RP11‐96L6 and RP13‐401N8. Thus, i(20q−) could be more precisely described as a der(20)del(20)(q11q13)idic(20)(p11). © 2006 Wiley‐Liss, Inc.