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Identification of consistent novel submegabase deletions in low‐grade oligodendrogliomas using array‐based comparative genomic hybridization
Author(s) -
Rossi Michael R.,
Gaile Daniel,
LaDuca Jeffrey,
Matsui SeiIchi,
Conroy Jeffrey,
McQuaid Devin,
Chervinsky David,
Eddy Roger,
Chen HaiShen,
Barnett Gene H.,
Nowak Norma J.,
Cowell John K.
Publication year - 2005
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.20218
Subject(s) - comparative genomic hybridization , biology , genetics , genome , chromosome , gene , microbiology and biotechnology
We have analyzed 18 low‐grade gliomas using array comparative genomic hybridization (aCGH) with an average resolution of <500 kb. Because the majority of these tumors showed loss of chromosome arms 1p and 19q, we used custom statistical approaches to define submegabase hemizygous losses throughout the genome that correlated with 19q loss. As a result of this analysis, we have identified a ∼550‐kb region in 11q13 and a ∼300‐kb region in 13q12 that showed hemizygous deletion in virtually all the tumors analyzed regardless of their 1p/19q status. FISH analyses of interphase nuclei from the same tumors used for aCGH analysis confirmed the hemizygous loss. The identification of such specific changes provides a potentially very useful diagnostic marker for this subgroup of low‐grade tumors. These regions of the genome define small numbers of candidate genes that are within the deletions. The aCGH analysis also defined the spectrum of gain and loss of genomic regions in low‐grade oligodendrogliomas. © 2005 Wiley‐Liss, Inc.