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Identification of recurrent chromosomal aberrations in germ cell tumors of neonates and infants using genomewide array‐based comparative genomic hybridization
Author(s) -
Veltman Imke,
Veltman Joris,
Janssen Irene,
Hulsbergenvan de Kaa Christina,
Oosterhuis Wolter,
Schneider Dominik,
Stoop Hans,
Gillis Ad,
Zahn Susanne,
Looijenga Leendert,
Göbel Ulrich,
van Kessel Ad Geurts
Publication year - 2005
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.20208
Subject(s) - comparative genomic hybridization , biology , germ cell tumors , yolk sac , chromosome , genetics , pathology , oncology , gene , embryo , medicine , chemotherapy
Abstract Human germ cell tumors (GCTs) of neonates and infants comprise a heterogeneous group of neoplasms, including teratomas and yolk sac tumors with distinct clinical and epidemiologic features. As yet, little is known about the cytogenetic constitution of these tumors. We applied the recently developed genomewide array‐based comparative genomic hybridization (array CGH) technology to 24 GCTs derived from patients under the age of 5 years. In addition, we included seven tumors derived from children and adolescents older than 5 years. In the series from those under the age of 5 years, most teratomas displayed normal profiles, except for some minor recurrent aberrations. In contrast, the yolk sac tumors displayed recurrent losses of 1p35–pter and gains of 3p21–pter and of 20q13. In the GCTs of patients older than 5 years, the main recurrent anomalies included gains of 12p and of whole chromosomes 7 and 8. In addition, gains of the 1q32–qter region and losses of the 6q24–qter and 18q21–qter regions were frequent in GCTs of varied histology, independent of age. We concluded that array CGH is a highly suitable method for identifying recurrent chromosomal anomalies in GCTs of neonates and infants. The recurrent anomalies observed point to chromosomal regions that may harbor novel diagnostic/prognostic identifiers and genes relevant to the development of these neoplasms. © 2005 Wiley‐Liss, Inc.