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High‐resolution chromosome arm 5p array CGH analysis of small cell lung carcinoma cell lines
Author(s) -
Coe Bradley P.,
Henderson LauraJane,
Garnis Cathie,
Tsao MingSound,
Gazdar Adi F.,
Minna John,
Lam Stephen,
MacAulay Calum,
Lam Wan L.
Publication year - 2005
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.20137
Subject(s) - comparative genomic hybridization , biology , chromosome , breakpoint , clone (java method) , genetics , bacterial artificial chromosome , small cell lung carcinoma , gene , genome , carcinoma , small cell carcinoma
Abstract Genomic amplification of regions on chromosome arm 5p has been observed frequently in small cell lung cancer (SCLC), implying the presence of multiple oncogenes on this arm. Although conventional comparative genomic hybridization (CGH) detects gross chromosomal copy number changes, gene discovery requires a higher‐resolution approach in order to identify regions of alteration precisely. To identify candidate genes on this chromosome arm, we developed a high‐resolution, 10‐clone‐per‐megabase bacterial artificial chromosome CGH array for 5p and examined a panel of 15 SCLC cell lines. Utilization of this CGH array has allowed the fine‐mapping of breakpoints to regions as small as 200 kb in a single experiment. In addition to reporting our observations of aberrations at the well‐characterized SKP2 and TERT loci, we describe the identification of microdeletions that have escaped detection by conventional screens and the identification TRIO and ANKH as novel putative oncogenes. © 2004 Wiley‐Liss, Inc.