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Differentially amplified chromosome 12 sequences in low‐ and high‐grade osteosarcoma
Author(s) -
Gisselsson David,
Pålsson Eva,
Höglund Mattias,
Domanski Henryk,
Mertens Fredrik,
Pandis Nikos,
Sciot Raf,
Dal Cin Paola,
Bridge Julia A.,
Mandahl Nils
Publication year - 2002
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.1219
Subject(s) - ring chromosome , biology , chromosome , metaphase , fluorescence in situ hybridization , chromosome 12 , microbiology and biotechnology , karyotype , genetics , cytogenetics , chromosome 19 , chromosome 3 , gene
Most osteosarcomas are highly aggressive malignancies characterized by a complex pattern of chromosome abnormalities. However, a subgroup of low‐grade, parosteal tumors exhibits a relatively simple aberration pattern dominated by ring chromosomes carrying amplified material from chromosome 12. To assess whether sequences from this chromosome were differentially amplified in low‐ and high‐grade osteosarcomas, copy numbers of the CCND2, ETV6, KRAS2, and D12S85 regions in 12p and the MDM2 region in 12q were evaluated by interphase or metaphase fluorescence in situ hybridization (FISH) in 24 osteosarcomas. Amplification of MDM2 was detected in all five low‐grade and four high‐grade osteosarcomas, all of which showed ring chromosomes. An overrepresentation of 12p sequences was found in 1/5 low‐grade and in 9/19 high‐grade tumors. Multicolor single‐copy FISH analysis of metaphase cells from six high‐grade tumors showed that extra 12p material either occurred together with MDM2 in ring chromosomes or was scattered over the genome as a result of complex structural rearrangements. Most tumors (8/10) not containing amplification of the assessed chromosome 12 loci exhibited a nondiploid pattern at evaluation with probes for centromeric alpha satellite sequences. These findings indicate that gain of sequences from the short arm of chromosome 12 could be a possible genetic pathway in the development of aggressive osteosarcoma. © 2002 Wiley‐Liss, Inc.