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Genome‐wide analysis of sixteen chordomas by comparative genomic hybridization and cytogenetics of the first human chordoma cell line, U‐CH1
Author(s) -
Scheil Stefanie,
Brüderlein Silke,
Liehr Thomas,
Starke Heike,
Herms Jochen,
Schulte Michael,
Möller Peter
Publication year - 2001
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.1184
Subject(s) - chordoma , comparative genomic hybridization , biology , fluorescence in situ hybridization , molecular cytogenetics , cytogenetics , karyotype , genetics , gene , chromosome , pathology , medicine
Cytogenetic information on chordomas is rudimentary and restricted to GTG‐banding analysis of 26 cases worldwide. In this study, we present the chromosomal imbalances detected in a series of 16 chordomas (10 sacrococcyeal, five sphenooccipital, and one spinal) from 13 patients using comparative genomic hybridization (CGH) and fluorescence in situ hybridization (FISH). On average, 3.2 losses and 4.2 gains were detected per tumor. The most common DNA copy number alterations were losses on chromosomal arms 3p (50%) and 1p (44%). Losses of 3p were detected in five of seven primary chordomas. Therefore, the loss of 3p might be an early event in chordoma genesis. The most common gains involved 7q (69%), 20 (50%), 5q (38%), and 12q (38%). Additionally, we raised the first human chordoma cell line, U‐CH1, from a recurrence of a sacral chordoma. U‐CH1 and its parent tumor had almost the same CGH profile. According to GTG‐banding and multicolor FISH, U‐CH1 has the following clonal chromosomal abnormalities: der(1)t(1;22), del(4), +del(5), +del(6), +7, del(9), del(10), +der(20)t(10;20), +21. Thus, the novel permanent human chordoma cell line U‐CH1 has chordoma‐typical cytogenetic aberrations. Our data suggest that tumor suppressor genes or mismatch repair genes (located at 1p31 and 3p14) and oncogenes (located in 7q36) might be involved in chordoma genesis. © 2001 Wiley‐Liss, Inc.