Premium
Analysis of loss of heterozygosity in lymphoma and leukaemia arising in F1 hybrid mice locates a common region of chromosome 4 loss
Author(s) -
Meijne Emmy,
Huiskamp Rene,
Haines Jackie,
Moody John,
Fin Rosemary,
Wilding Jennifer,
Spanjer Sylvia,
Bouffler Simon,
Edwards Alan,
Cox Roger,
Silver Andrew
Publication year - 2001
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.1156
Subject(s) - loss of heterozygosity , biology , chromosome , genetics , tumor suppressor gene , lymphoma , allele , microsatellite , gene , cancer research , carcinogenesis , immunology
Previous studies have identified five lymphoma‐related tumour suppressor gene regions on murine chromosome 4. Using detailed allelotype analysis on a range of lympho‐haematopoietic tumour types arising in F1 hybrid mice, we now show a consistent pattern of loss of heterozygosity (LOH) which identifies a common region of loss delineated by microsatellites D4Mit21 and D4Mit53 on proximal chromosome 4. This critical segment corresponds to the thymic lymphoma tumour suppressor region 5 (TLSR5) identified in an earlier study. Tumours of this type have also been reported as showing allelic loss from the Trp53 and Ikaros regions on chromosome 11. In the present study, only a small fraction of tumours showed LOH in the Ikaros region, while a minority of lymphomas, but not acute myeloid leukaemias, showed allelic loss of the chromosome 11 segment encoding Trp53 . These and other data indicate strongly that the genomic regions identified as showing recurrent LOH depend on the genetic background of the mice. Overall, the results indicate a key role for a tumour suppressor gene(s) encoded in an ∼3 cM segment on proximal chromosome 4 and provide an experimental basis for the further investigation of the functional role of candidate genes which include Pax5 and Tgfbr1 . © 2001 Wiley‐Liss, Inc.