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A classification efficiency test of spectral karyotyping and multiplex fluorescence in situ hybridization: Identification of chromosome homologies between Homo sapiens and Hylobates leucogenys
Author(s) -
Rens Willem,
Yang Fengtang,
O'Brien Patricia C. M.,
Solanky Nita,
FergusonSmith Malcolm A.
Publication year - 2001
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.1119
Subject(s) - fluorescence in situ hybridization , karyotype , hylobates , biology , multiplex , homo sapiens , chromosome , in situ , genetics , fish <actinopterygii> , evolutionary biology , zoology , gene , physics , sociology , fishery , meteorology , anthropology
Two digital fluorescence microscopy systems, spectral karyotyping (SKY) and multiplex fluorescence in situ hybridisation (M‐FISH), are used with multicolour probe sets to assist in the detection of chromosome aberrations. We have compared the resolution of the two methods in their ability to identify karyotype rearrangements, which have occurred during the divergence of Homo sapiens and Hylobates leucogenys in evolution. A 24‐color human paint kit distinguishes 74 conserved autosomal segments in H. leucogenys , some of which are difficult to resolve. We examined the extent to which the SKY and M‐FISH techniques are able to detect the smallest of these bands. We have found this to be a rigorous test of multicolour chromosome classification systems. We conclude from our results that both systems are able invariably to classify the majority of conserved segments but differ in the efficiency of detection of small inserts. © 2001 Wiley‐Liss, Inc.