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Detailed gene copy number and RNA expression analysis of the 17q12–23 region in primary breast cancers
Author(s) -
Willis Simon,
Hutchins AnneMarie,
Hammet Fleur,
Ciciulla John,
Soo WeeKheng,
White David,
van der Spek Peter,
Henderson Michael A.,
Gish Kurt,
Venter Deon J.,
Armes Jane E.
Publication year - 2003
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.10138
Subject(s) - biology , gene , comparative genomic hybridization , wnt signaling pathway , carcinogenesis , candidate gene , gene duplication , cancer research , breast cancer , genetics , chromosome , cancer
Chromosome region 17q12–23 commonly shows an increase in DNA copy number in breast cancers, suggesting that several oncogenes are located at this site. We performed a high‐resolution expression array and comparative genomic hybridization analysis of genes mapped to the entire 17q12–23 region, to identify novel candidate oncogenes. We identified 24 genes that showed significant overexpression in breast cancers with gain of 17q12–23, compared to cancers without gain. These genes included previously identified oncogenes, together with several novel candidate oncogenes. FISH analysis using specific gene probes hybridized to tissue arrays confirmed the underlying amplification of overexpressed genes. This high‐resolution analysis of the 17q12–23 region indicates that several established and novel candidate oncogenes, including a Wnt‐signaling pathway member, are amplified and overexpressed within individual primary breast cancer samples. We were also able to confirm the presence of two apparently separate and reciprocally amplified groups of genes within this region. Investigation of these genes and their functional interactions will facilitate our understanding of breast oncogenesis and optimal management of this disease. © 2003 Wiley‐Liss, Inc.