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Breakpoint characterization of der(9) deletions in chronic myeloid leukemia patients
Author(s) -
Storlazzi Clelia T.,
Specchia Giorgina,
Anelli Luisa,
Albano Francesco,
Pastore Domenico,
Zagaria Antonella,
Rocchi Mariano,
Liso Vincenzo
Publication year - 2002
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.10116
Subject(s) - derivative chromosome , breakpoint , chromosomal translocation , myeloid leukemia , biology , chromosome , genetics , microbiology and biotechnology , chromosome 22 , gene , cancer research
Deletions adjacent to the 9/22 translocation breakpoint on the derivative chromosome 9 have recently been described in a substantial number of chronic myeloid leukemia (CML) cases, but their extension has not been characterized in detail. Using FISH with an appropriate set of BAC/PAC probes, we have characterized the deletion in 10 CML cases, identified by screening 71 patients at diagnosis. Five patients showed a complex chromosome rearrangement and 3 of them were deleted. The size of the deletion was variable, ranging from few hundreds kb to 8 Mb. A minimally deleted region on both chromosomes 9 and 22 was identified and was found to contain the ASS gene on chromosome 9 and IGLL1 on chromosome 22. © 2002 Wiley‐Liss, Inc.