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Cryopreserved chronic lymphocytic leukemia cells analyzed by multicolor fluorescence in situ hybridization after optimized mitogen stimulation
Author(s) -
Karhu Ritva,
Vilpo Leena,
Isola Jorma,
Knuutila Sakari,
Vilpo Juhani
Publication year - 2002
Publication title -
genes, chromosomes and cancer
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.754
H-Index - 119
eISSN - 1098-2264
pISSN - 1045-2257
DOI - 10.1002/gcc.10074
Subject(s) - fluorescence in situ hybridization , interphase , chronic lymphocytic leukemia , in situ , cryopreservation , in situ hybridization , chromosomal translocation , biology , leukemia , microbiology and biotechnology , cancer research , chromosome , immunology , chemistry , genetics , gene , embryo , gene expression , organic chemistry
We investigated the utility of multicolor in situ fluorescence hybridization (mFISH) on cryopreserved blood cells from 11 chronic lymphocytic leukemia (CLL) patients. The results demonstrate that an individually chosen optimized mitogen combination induces proliferation of neoplastic B‐cells after cryopreservation. Abnormal cells were detected in eight samples by mFISH, and, in six samples, the abnormality could be verified by comparative genomic hybridization or interphase FISH. In addition to typical CLL abnormalities, such as del(11q) or +12, several balanced translocations and single‐cell abnormalities were found. Thus, mFISH can reveal new prognostically relevant chromosome aberrations in CLL. © 2002 Wiley‐Liss, Inc.