Open AccessDevelopment and optimization of an immunoassay for the detection of Hg(II) in lake water
Author(s) -
Jin Xuexue,
He Rong,
Ju Xingrong,
Zhang Jie,
Wang Mingjie,
Xing Changrui,
Yuan Jian
Publication year - 2019
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.991
Subject(s) - polyvinyl alcohol , tap water , detection limit , immunoassay , chemistry , chromatography , bovine serum albumin , mercury (programming language) , gelatin , pulmonary surfactant , metal ions in aqueous solution , monoclonal antibody , metal , antibody , biochemistry , environmental engineering , biology , environmental science , organic chemistry , computer science , immunology , programming language
In this paper, an indirect competitive enzyme‐linked immunosorbent assay (IC‐ELISA) has been developed and optimized to detect Hg(II) in tap water and lake water based on a monoclonal antibody (mAb‐A24). Some stabilizing additives (Gelatin, bovine serum albumin [BSA], polyvinyl alcohol [PVA], and polyvinyl pyrrolidone [PVP]) and surfactant (Tween‐20) have been investigated thoroughly in the optimization process. Under the optimal condition, the 50% half maximal inhibitory concentration (IC 50 ) and limit of detection (LOD) were 1.68 and 0.079 ng/ml, respectively. These anti‐Hg mAbs also have some affinity with methyl mercury (CH 3 Hg) and with no cross‐reactivity with other thirteen metal ions. The developed method has shown satisfactory recovery of Hg(II), ranged between 91% and 116%, from tap water and lake water. Therefore, this immunoassay can be used to detect trace Hg(II) in environment water.