Open AccessAmebicidal effects of fenugreek ( Trigonella foenum‐graecum ) against Acanthamoeba cysts
Author(s) -
Kaya Yasemin,
Baldemir Ayşe,
Karaman Ülkü,
Ildız Nilay,
Arıcı Yeliz Kasko,
Kaçmaz Gamze,
Kolören Zeynep,
Konca Yusuf
Publication year - 2019
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.849
Subject(s) - trigonella , hemocytometer , traditional medicine , acanthamoeba , biology , in vivo , pharmacology , toxicology , chemistry , veterinary medicine , microbiology and biotechnology , medicine , biochemistry
Trigonella foenum‐graecum L. ( TF ) is known to the public as a chest emollient, mucous expectorant, laxative and is used to prevent maturation of boils and diabetes since ancient times. In this study, we aimed to determine the amebicidal effects against Acanthamoeba cysts. Plant extracts were prepared at concentrations of 1, 2, 4, 8, 16, and 32 mg/ml and were placed in a hemocytometer with cell counts 22 × 10 6 cell/ml. The fatty acid profiles of TF seeds were determined. Standard Acanthamoeba cysts were added and incubated at 25°C. The viability of the parasite was checked and recorded at hours 3, 24, 48, 72, 96, and 102. The values of lethal concentration doses ( LD 50 and LD 90) were calculated using probit analysis. This study revealed that T. foenum‐graecum prevented proliferation of the parasite at certain times. However, further for in vivo and controlled experimental studies are needed in order to find out how to use this plant as medication.