Open AccessExtraction, purification, and structure characterization of polysaccharides from Crassostrea rivularis
Author(s) -
Qin Xiaoming,
Fan Xiuping,
Zhang Lianyi,
Zheng Huina,
Zhang Chaohua,
Yuan Jianjun
Publication year - 2018
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.695
Subject(s) - chemistry , chromatography , gel permeation chromatography , polysaccharide , rhamnose , column chromatography , cellulose , sodium hydroxide , sepharose , potassium hydroxide , biochemistry , organic chemistry , polymer , enzyme
Crude polysaccharide was prepared from Crassostrea rivularis by 30% (w/v) potassium hydroxide solution at 90°C for 120 min. Three fractions ( OG 1, OG 2, and OG 3) were purified by DEAE ‐52 cellulose and Sepharose 2B gel column chromatography. The chemical structures were determined using gas chromatography ( GC ), high‐performance gel permeation chromatography ( HPGPC ), Fourier‐transform infrared ( FT ‐ IR ) spectroscopy, and 1 H and 13 C nuclear magnetic resonance ( NMR ) spectroscopy. The results indicated that OG 1 was composed of rhamnose and little mannose (8.71%), the ratio of Rha: Gal: Xyl: Fuc in OG 3 were 14:5.5:3:1. And their average molecular weights (Mw) were about 1.66 × 10 6 and 2.33 × 10 6 Da, respectively. OG 2 was composed only of glucose (98.23%), which means it was glycogen. OG 2 was consisted mainly of →4)‐ α‐D‐Glc‐(1→, with the branch chain every 6.5 glucose residues on average, which is →4,6)‐α‐D‐Glc‐(1→ and trace amount of α‐D‐Glc‐(1→ branched units. The Mw was 2.27 × 10 6 Da. It provides the bases for the bioactivity research.