Open AccessEvaluation of chemical components in Citri Reticulatae Pericarpium of different cultivars collected from different regions by GC – MS and HPLC
Author(s) -
Luo Meixia,
Luo Hujie,
Hu Pingjun,
Yang Yiting,
Wu Bo,
Zheng Guodong
Publication year - 2018
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.569
Subject(s) - hesperidin , nobiletin , chemistry , cultivar , limonene , linalool , chromatography , high performance liquid chromatography , myrcene , rutaceae , essential oil , botany , flavonoid , biology , biochemistry , medicine , alternative medicine , pathology , antioxidant
To discriminate the feasible differences and find potential similarities and relationships of Citri Reticulatae Pericarpium ( CRP ), this work was accomplished by a comprehensive and reliable method using gas chromatography–mass spectrometer ( GC – MS ) to analyze the volatile oils and high‐performance liquid chromatography ( HPLC ) simultaneously to determine the contents of five bioactive flavonoids, namely hesperidin, nobiletin, 3,5,6,7,8,3′,4′‐heptamethoxyflavone, tangeretin, and 5‐hydroxy‐6,7,8,3′,4′‐pentamethoxyflavone in 25 batches of CRP samples of 10 cultivars collected from different regions in China. The GC – MS analyses indicated that 98 compounds were successfully identified from the volatile oils obtained and the major constituents of volatile oil are d ‐limonene, γ‐terpinene, α‐pinene, linalool, and myrcene. Even 2‐(methylamino) benzoate was found in all cultivar samples harvested at maturation stage. Under the optimal condition, the quantitative analyses of five bioactive flavonoids were successfully performed by HPLC and hierarchical cluster analysis ( HCA ). Results showed significant differences among cultivars in the contents of five bioactive flavonoids mentioned earlier. The HCA and GC – MS results provided a convenient approach which might be applied for rapid similarity evaluation and also holds the potential for analysis of compounds present in other plants. Therefore, this work obtained offers scientific basis to control quality and develop medicinal value of the medicinal materials in CRP .