Open AccessGenistein suppresses the proliferation of telomerase‐negative cells
Author(s) -
Lin ChuanChuan,
Hsieh MengHsun,
Teng ShuChun
Publication year - 2017
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.382
Subject(s) - telomerase , genistein , telomere , plasmid , microbiology and biotechnology , yeast , biology , chemistry , dna , genetics , gene
In both tumor and yeast cells that lack telomerase, telomeres are maintained via an alternative recombination mechanism. In this study, we tested genistein, a potential TOP 2 inhibitor required for telomere–telomere recombination, on the repression of telomere–telomere recombination. Genistein on the repression of type II recombination on a tlc1 yeast strain was examined by the telomeric DNA structures using Southern blot analysis. Telomere patterns of freshly dissected tlc1 spores containing an empty plasmid ( pYES 2) or a yeast TOP 2 ( yTOP 2) plasmid were analyzed. The results indicated that the reintroduction of TOP 2 recovered the type II pattern, implying genistein in the blockage of type II survivors in the tlc1 strain. The effects of genistein on both tlc 1 and tlc1 rad 51 strains in liquid and solid mediums were also examined. Finally, treatment of 10 μ mol/L of genistein showed inhibitory effect on the growth of telomerase‐negative U2 OS alternative lengthening of telomere ( ALT ) cells, but not in telomerase‐positive HCT 116 cells. These results provide evidences that the inhibitory effects of genistein on telomerase‐negative cells depend on type II recombination pathway in yeast and the ALT pathway in human tumors.