Open AccessAntioxidant activities of essential oil of Bidens pilosa (Linn. Var. Radita) used for the preservation of food qualities in North Cameroon
Author(s) -
Goudoum Augustin,
Abdou Armand B.,
Ngamo Léonard Simon T.,
Ngassoum Martin Benoît,
Mbofung Carl M. F.
Publication year - 2016
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.330
Subject(s) - dpph , chemistry , antioxidant , essential oil , carotene , food science , bidens pilosa , botany , biology , organic chemistry
This study aimed to determine the total antioxidant capacity of the essential oil (EO) of leaves of Bidens pilosa (Linn. Var. Radita ) used as protectant of stored grains in Northern Cameroon. EO was characterized by GC‐FID, antioxidant activity (AA) was determined by combining: evaluation of radical‐scavenging activity, reducing power (RP) and co‐oxidation of β ‐carotene methods. Tests were carried out on crude and stored EO kept for two weeks at 31.48 ± 2.88°C and 58.56 ± 6.78% relative humidity. These conditions are the same as those of grain storage. GC analyses enabled the identification of 27 compounds, representing around 97.57% of the total oil contents. The major constituents of the EO were α ‐pinene (14.7%), ε ‐caryophyllene (13.5), and β ‐ocimene (12.8%). The AA of the crude and stored EO are proportional to the concentrations and time of exposition. Exposed at the day light, this EO inhibit 77.4–18.69% for the DPPH system, 59.55–19.14% for RP method and 91.88–21.8% for β ‐carotene‐linoleate model system, respectively, from crude and 15 days storage EO at 20 mg L −1 . For the EC 50 values, β ‐carotene method is excellent and in the decreasing order of DPPH method, PR with 2.52 mg L −1 , 2.77 mg L −1 and 4.13 mg L −1 , respectively, for the crude oil. The ET 50 were 1.59 days for the RP method and 2.88 days DPPH system and β ‐carotene‐linoleate model system at 20 mg L −1 . These results showed that the EO of B. pilosa leaves exhibits AA that might be an added value for this EO preventing stored products from pest attacks.