Open AccessProbiotics encapsulated gastroprotective cross‐linked microgels: Enhanced viability under stressed conditions with dried apple carrier
Author(s) -
Afzaal Muhammad,
Saeed Farhan,
Ateeq Huda,
Akhtar Muhammad Nadeem,
Imran Ali,
Ahmed Aftab,
Aamir Muhammad,
Islam Fakhar,
Yasmin Iqra,
Shah Yasir Abbas,
Hussain Muzzamal,
Hameed Adnan,
Kumar Roshan,
Awuchi Chinaza Godswill
Publication year - 2023
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.3116
Subject(s) - lactobacillus acidophilus , whey protein isolate , probiotic , food science , chemistry , viability assay , whey protein , microbiology and biotechnology , bacteria , biochemistry , biology , in vitro , genetics
In the current study, Lactobacillus acidophilus was encapsulated in sodium alginate and whey protein isolate, with the addition of antacids CaCO 3 or Mg(OH) 2 . The obtained microgels were observed by scanning electron microscopy. Encapsulated and free probiotics were subjected to vitality assay under stressed conditions. Furthermore, dried apple snack was evaluated as a carrier for probiotics for 28 days. A significant ( p ≤ .05) effect of antacid with an encapsulating agent was observed under different stressed conditions. During exposure to simulated gastrointestinal conditions, there were observations of 1.24 log CFU and 2.17 log CFU, with corresponding 0.93 log CFU and 2.63 log CFU decrease in the case of SA + CaCO 3 and WPI + CaCO 3 respectively. Likewise, high viability was observed under thermal and refrigerated conditions for probiotics encapsulated with SA + CaCO 3 . In conclusion, the results indicated that alginate microgels with CaCO 3 are effective in prolonging the viability of probiotics under stressed conditions.