Open AccessDevelopment of an ultrasensitive molecularly imprinted poly‐(ortho‐phenylenediamine) based sensor for the determination of melamine adulteration in milk and infant formula
Author(s) -
HeydarianDehkordi Negin,
SaeiDehkordi Seyyed Siavash,
Izadi Zahra,
GhasemiVarnamkhasti Mahdi
Publication year - 2022
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.2914
Subject(s) - melamine , detection limit , repeatability , graphene , electrode , infant formula , materials science , electrochemical gas sensor , cyclic voltammetry , molecularly imprinted polymer , colloidal gold , molecular imprinting , reproducibility , nuclear chemistry , nanoparticle , chromatography , chemistry , nanotechnology , electrochemistry , organic chemistry , selectivity , catalysis , biochemistry , composite material
A sensitive molecularly imprinted poly‐(ortho‐phenylenediamine) electrochemical sensor was fabricated for selective melamine detection in milk and infant formula. The pencil graphite electrode (PGE) was modified by deposition of Au nanoparticles and reduced graphene oxide (RGO) on its surface. The fabrication of the electrode in various stages was monitored using cyclic voltammetry. The immobilized RGO, MIP, and gold nanoparticles on the PGE surface were morphologically characterized by field‐emission scanning electron microscopy (FESEM). Under the optimized conditions, the linear range and the limit of detection (LOD) were 10 –17 –10 –8 M and 2.64 × 10 –16 M (S/N = 3), respectively. The prepared sensor exhibited a good reproducibility and repeatability response. The recovery range of melamine‐spiked milk and infant formula was 92.7%–103.9% and 93.5%–105.8%, respectively. The sensor could apply successfully for melamine determination in milk and infant formula samples.