Open AccessAntibacterial interactions of pulegone and 1,8‐cineole with monolaurin ornisin against Staphylococcus aureus
Author(s) -
Farhanghi Ali,
Aliakbarlu Javad,
Tajik Hossein,
Mortazavi Negar,
Manafi Leila,
JalilzadehAmin Ghader
Publication year - 2022
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.2870
Subject(s) - staphylococcus aureus , nisin , pulegone , antibacterial activity , minimum inhibitory concentration , chemistry , minimum bactericidal concentration , microbiology and biotechnology , antibacterial agent , bacteria , food science , biology , antibiotics , antimicrobial , essential oil , genetics
The aim of this study was to investigate the antibacterial interactions of pulegone and 1,8‐cineole with monolaurin ornisin against Staphylococcus aureus . The individual and combined antibacterial activities of the compounds were evaluated using minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), fractional inhibitory concentration index (FICi), and time‐kill methods. Furthermore, the mechanism of the antibacterial action of the compounds was tested by measuring the release of cell constituents. The MIC values of pulegone, 1,8‐cineole, nisin, and monolaurin were 5.85 µl/ml, 23.43 µl/ml, 6.25 µg/ml, and 0.031 mg/ml, respectively. A synergistic antibacterial activity (FICi = 0.5) was found between 1,8‐cineole and nisin. The time‐kill assay showed that the populations of S. aureus exposed to 1,8‐cineole, nisin, and their combination were decreased by 5.9, 5.3, and 7.1 log CFU (colony‐forming units)/mL, respectively. The combination of 1,8‐cineole and nisin also induced the highest release of cell constituents. It was concluded that the combination of 1,8‐cineole and nisin could be considered as a novel and promising combination which may reduce the required dose of each antibacterial compound.