Open AccessQualitative and quantitative analyses of chemical components of Citri Sarcodactylis Fructus from different origins based on UPLC–Q–Exactive Orbitrap–MS and GC–MS
Author(s) -
Ning Jinrong,
Wang Kanghui,
Yang Wanling,
Liu Mengshi,
Tian Jingyuan,
Wei Minyan,
Zheng Guodong
Publication year - 2022
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.2822
Subject(s) - nobiletin , orbitrap , hesperidin , chromatography , chemistry , principal component analysis , mass spectrometry , scopoletin , coumarin , quantitative analysis (chemistry) , high performance liquid chromatography , flavonoid , biochemistry , organic chemistry , artificial intelligence , medicine , alternative medicine , pathology , computer science , antioxidant
Ultra‐high‐performance liquid chromatography–Q–Exactive Orbitrap–mass spectrometry (MS) and gas chromatography (GC)–MS were performed for the qualitative and quantitative analyses of Citri Sarcodactylis Fructus (CSF) from different origins. The contents of eight major CSF components, namely 5,7‐dimethoxycoumarin, scopoletin, hesperidin, tangeretin, nobiletin, limonin, nomilin, and stachydrine, were quantitatively analyzed. Clustering analysis and principal component analysis (PCA) were, respectively, performed to classify and compare the 10 CSF batches. One hundred and two volatile components were identified accordingly by comparing retention times, reference standards, parent peaks, fragment peaks, and findings from relevant literature. Moreover, high content of 5,7‐dimethoxycoumarin and stachydrine was detected in all the CSFs, especially in CSF‐Zhe. Therefore, the high content component coumarin “5,7‐dimethoxycoumarin” was suggested to be quality analysis component rather than hesperidin. Additionally, characteristic compounds were found to distinguish different CSFs. This work was a comprehensive study about the components of various CSF. It distinguished the basic differences in the compositions of CSF from different origins. Eventually, it provided experimental and systematic bases for the quality control analysis of CSF, which has potential application in the further research.