Open AccessImmunomodulatory effects of the polysaccharide from Sinonovacula constricta on RAW264.7 macrophage cells
Author(s) -
Liu Zhidong,
Liu Zhifang,
Li Laihao,
Zhang Junjie,
Zhao Qiancheng,
Lin Na,
Zhong Wenzhu,
Jiang Mei
Publication year - 2022
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.2735
Subject(s) - polysaccharide , mannose , western blot , macrophage , secretion , microbiology and biotechnology , chemistry , biology , biochemistry , gene , in vitro
This study aimed to evaluate the immunomodulatory effect of the polysaccharide from Sinonovacula constricta (SCP‐1‐1) in RAW264.7 cells. SCP‐1‐1 with a molecular weight of 440.0 kDa consisted of glucose and mannose. The immunomodulatory assay results showed that SCP‐1‐1 could significantly enhance phagocytic ability, NO production, and some cytokines (TNF‐α, IL‐6, and IL‐1β) secretion of RAW264.7 cell in a dose‐dependent manner. Western blot analysis results demonstrated that SCP‐1‐1 could regulate the expression levels of the key proteins in the signaling pathways of RAW264.7 cell and might associated with NF‐κβ and PI3K signaling pathway. These findings could contribute to elucidate the immunomodulatory activities of the polysaccharide from Sinonovacula constricta .