Open AccessImproved triplex real‐time PCR with endogenous control for synchronous identification of DNA from chicken, duck, and goose meat
Author(s) -
Liu GuoQiang,
Luo JianXing,
Xu WeiLiang,
Li ChunDong,
Guo YuanSheng,
Guo Liang
Publication year - 2021
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.2272
Subject(s) - goose , dna , endogeny , biology , real time polymerase chain reaction , polymerase chain reaction , positive control , microbiology and biotechnology , gene , biochemistry , medicine , paleontology , traditional medicine
The authentication and labeling of meat products, concerning origins and species, are key to fair trade and to protect consumer interests in the market. We developed an improved triplex real‐time PCR approach to simultaneously identify chicken, duck, and goose DNA in meat, including an endogenous control to avoid false negatives. Our method specifically detected DNA from chicken, duck, and goose, and showed no cross‐reaction with DNA extracted from other meat types. The detection limits of chicken, duck, and goose DNA were 0.001–0.00025 ng, 0.0025–0.0001 ng, and 0.001–0.00001 ng, respectively, and we were able to simultaneously identify DNA from two distinct origins using as little as 0.1% of total meat weight. Our newly generated triplex real‐time PCR method with endogenous control exhibited high specificity, sensitivity, and efficiency for simultaneous identification of DNA from chicken, duck, and goose in meat.