Open AccessAntibacterial, anti‐inflammatory, analgesic, and hemostatic activities of Acanthopanax trifoliatus (L.) merr
Author(s) -
Chen Zefeng,
Cheng Shupeng,
Lin Huiqiong,
Wu Wenjing,
Liang Liyi,
Chen Xicai,
Zheng Xi,
He Yan,
Zhang Kun
Publication year - 2021
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.2190
Subject(s) - chemistry , petroleum ether , ethyl acetate , chlorogenic acid , chloroform , polyphenol , ingredient , flavonoid , rutin , nutraceutical , active ingredient , anti inflammatory , analgesic , food science , traditional medicine , chromatography , extraction (chemistry) , antioxidant , biochemistry , pharmacology , biology , medicine
Acanthopanax trifoliatus (L.) Merr (AT) is a medicinal and edible plant with high nutritional value. The biological activity of A. trifoliatus (L.) Merr and its basis for injury treatment are explored in this paper. AT was ethanol‐extracted then refined separately with petroleum ether, chloroform, ethyl acetate, and n‐butanol. Active ingredients were analyzed, and anti‐bacterial, anti‐inflammatory, analgesic, and hemostatic effects were explored. Petroleum ether layer (PEL) extract produced the strongest antibacterial effect. Ethyl acetate layer (EAL) extract had the highest active substance content, with strong hemostatic and analgesic activities. Chloroform layer (CL) extract had the strongest anti‐inflammatory effect and significantly reduced IL‐1β protein expression. Active ingredients were analyzed using HPLC and UPLC‐MS to determine saponin, polyphenol, flavonoid, and characteristic ingredient contents. EAL extract had the highest polyphenol and flavonoid levels, including rutin, chlorogenic acid, isochlorogenic acid A, and isochlorogenic acid C, which may contribute to its nutritional activities. The study provides a reliable theoretical and practical basis for the applications of AT nutraceutical products.