Open AccessExtraction optimization by using response surface methodology and purification of yellow pigment from Gardenia jasminoides var. radicans Makikno
Author(s) -
Wu Jun,
Zhang Jiangtao,
Yu Xin,
Shu Yue,
Zhang Siyu,
Zhang Yinglao
Publication year - 2021
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.2046
Subject(s) - gardenia jasminoides , response surface methodology , chromatography , chemistry , extraction (chemistry) , absorbance , pigment , gardenia , desorption , crocin , adsorption , organic chemistry , biochemistry , traditional chinese medicine , medicine , alternative medicine , pathology
Abstract Gardenia jasminoides var. radicans Makikno contains rich gardenia yellow pigment (GYP). In this study, the process of pigment extraction was optimized based on a Box–Behnken design (BBD) and response surface methodology (RSM). The absorbance and antioxidant activity (AA) were considered as responses. The result showed that the optimal extraction conditions were ethanol concentration 65.10%, liquid/solid ratio 10:1 ml/g, extraction time 59.85 min, and extraction temperature 60.04℃ for the maximal response values of absorbance (0.79) and AA (91.30%), respectively. Crude GYP was purified by the 13 different resins. The result showed that BJ‐7514 was suitable for purifying GYP with the absorption ratio of 95.4%. Moreover, the 80% of ethanol eluent is applicable on the BJ‐7514 with the desorption ratio of 91.93%. The major component of GYP (Crocin‐3) was isolated and identified from the purified GYP.