Open AccessAnticancer property of Hemp Bioactive Peptides in Hep3B liver cancer cells through Akt/GSK3β/β‐catenin signaling pathway
Author(s) -
Wei LianHui,
Dong Yan,
Sun YuFeng,
Mei XueSong,
Ma XueSong,
Shi Jie,
Yang Qingli,
Ji YanRu,
Zhang ZhengHai,
Sun HuNan,
Sun XingRong,
Song ShuMin
Publication year - 2021
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.1976
Subject(s) - downregulation and upregulation , protein kinase b , cancer research , apoptosis , viability assay , cancer cell , signal transduction , liver cancer , cell culture , phosphorylation , cell , cancer , cell growth , chemistry , biology , microbiology and biotechnology , biochemistry , hepatocellular carcinoma , genetics , gene
Foodborne protein hydrolysates exhibit biological activity that may be therapeutic in a number of human disease settings. Hemp peptides (HP) generated by controlled hydrolysis of hemp proteins have a number of health benefits and are of pharmaceutical value. In the present study, we produce small molecular weight HP from hemp seed and investigate its anticancer properties in Hep3B human liver cancer cells. We demonstrate that HP treatment increased apoptosis, reduced cell viability, and reduced cell migration in Hep3B human liver cancer cells without affecting the normal liver cell line L02. We correlate these phenotypes with increased cellular ROS levels, upregulation of cleaved caspase 3 and Bad, and downregulation of antiapoptotic Bcl‐2. HP treatment led to increased Akt and GSK‐3β phosphorylation, with subsequent downregulation of β‐catenin, suggesting β‐catenin signaling modulation as a critical mechanism by which HP exhibits anticancer properties. Our findings suggest HP are of potential therapeutic interest for liver cancer treatment.