Open AccessGenotypic and antimicrobial resistance characterizations of Cronobacter sakazakii isolated from powdered milk infant formula: A comparison between domestic and imported products
Author(s) -
Pakbin Babak,
Mahmoudi Razzagh,
Mousavi Shaghayegh,
Allahyari Samaneh,
Amani Zahra,
Peymani Amir,
Qajarbeygi Peyman,
Hoseinabadi Zahra
Publication year - 2020
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.1965
Subject(s) - genotyping , biology , microbiology and biotechnology , ampicillin , genotype , cronobacter sakazakii , ciprofloxacin , multiple drug resistance , population , antibiotic resistance , infant formula , antimicrobial , tetracycline , drug resistance , antibiotics , food science , genetics , gene , medicine , environmental health
Cronobacter sakazakii , an opportunistic foodborne pathogen and a main cause of meningitis in neonates, is usually isolated from powdered milk infant formula (PMIF). At the present study, C. sakazakii were isolated from imported and domestically produced PMIF samples and identified by detection of ompA gene using real‐time PCR SYBR green melting curve following the evaluation of antimicrobial susceptibility and genotyping of the isolates employing BOX‐PCR and RAPD methods. We detected totally 5% contamination rate and a significantly higher prevalence of C. sakazakii in bulky imported domestically packaged PMIF samples. Also, our isolates were recognized as multidrug‐resistant pathogen completely resistant to ampicillin and amoxicillin; and intermediately resistant to ciprofloxacin and tetracycline antimicrobials. Genotype clustering patterns of bulky imported and imported product isolates were identical by both genotyping methods. Far genetic relatedness of domestic isolate to other isolates and the reference strain indicated higher genetic diversity of the domestic isolate genome. Multidrug resistance and diverse population genetic make complicated situation for determination of strategies for infectious disease prevention.