Open AccessSimultaneous detection of ovine and caprine DNA in meat and dairy products using triplex Taq Man real‐time PCR
Author(s) -
Guo Liang,
Yu Yuan,
Xu WeiLiang,
Li ChunDong,
Liu GuoQiang,
Qi Lemuge,
Luo JianXing,
Guo YuanSheng
Publication year - 2020
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.1936
Subject(s) - taqman , real time polymerase chain reaction , food science , dna , biology , biochemistry , gene
In this study, we report a new approach for the detection of ovine and caprine DNA in meat and dairy products using real‐time PCR protocol. Our new approach is based on the use of endogenous control and species‐specific Taq Man fluorescence probes. With this methodology, we specifically detected ovine and caprine DNA in meat and dairy products, with limits of detection of 0.001 ng and 0.01 ng for fresh and processed ovine meats, respectively, and 0.00025 ng, 0.005 ng, and 0.01 ng for caprine meat, milk, and cheese, respectively. Artificial meat and milk mixtures from sheep and goat were used to validate the protocol. Our results support that Taq Man real‐time PCR with endogenous control is an efficient and accurate method to detect DNA from sheep and goat in meat and dairy products.