Open AccessThe determination of lycopene Z ‐isomer absorption coefficient on C30‐HPLC
Author(s) -
Huang Jin,
Hui Bodi
Publication year - 2020
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.1879
Subject(s) - high performance liquid chromatography , chromatography , chemistry , lycopene , absorption (acoustics) , phase (matter) , chromatography detector , analytical chemistry (journal) , materials science , organic chemistry , composite material , antioxidant
Both E ‐ and Z ‐isomers of lycopene are encountered in nature. Although they were separated on C30‐HPLC by the mobile phase consisting of CH 3 CN‐MeOH (A) and MTBE (B), the quantification of Z ‐isomers cannot be archived at present because either their commercially available reference samples are currently lacking, or they are unstable. In this study, both the specific and molar absorption coefficients of 5, 9, and 13 Z ‐isomers in the mobile phase were determined on the analytical C30‐HPLC‐PDA‐ELSD, and further verified on the preparative C30‐HPLC‐PDA. The specific and molar absorption coefficients of 5, 9, and 13 Z ‐isomers were finally verified to be A 1 cm1 % = 3,422 and ε mol = 183,717, A 1 cm1 % = 2,183 and ε mol = 117,199, and A 1 cm1 % = 1,119 and ε mol = 60,076, respectively, in the mobile phase. With these determined coefficients, the quantifications of 5, 9, and 13 Z ‐isomers were able to be archived on C30‐HPLC‐PDA with the mobile phase applied in this investigation.