Open AccessThe protective effect of soybean protein‐derived peptides on apoptosis via the activation of PI3K‐AKT and inhibition on apoptosis pathway
Author(s) -
Yi Guofu,
Li He,
Li You,
Zhao Fen,
Ying Zhiwei,
Liu Menglan,
Zhang Jian,
Liu Xinqi
Publication year - 2020
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.1776
Subject(s) - mg132 , apoptosis , protein kinase b , pi3k/akt/mtor pathway , western blot , fas ligand , phosphorylation , microbiology and biotechnology , chemistry , flow cytometry , biology , programmed cell death , biochemistry , proteasome inhibitor , gene
Soybean protein‐derived peptides (SBP) are a rich source of various bioactive peptides with multiple health benefits. However, the prospective effects of SBP on human cells are still unclear. Therefore, this article investigated the effects of small molecular weight SBP on MG132‐induced apoptosis in RAW264.7 cells. SBP inhibited MG132‐induced apoptosis of RAW264.7 cells in a dose‐dependent manner by flow cytometry. To further study its molecular mechanisms, Western blot analysis demonstrated that SBP could activate the PI3K‐AKT pathway by increasing the phosphorylation of PI3K and AKT and inhibiting apoptosis pathway by downregulating the expressions of pro‐apoptotic proteins of Bim, Bax, Fas, and Fasl and promoting the expressions of anti‐apoptotic proteins of Bcl‐xL and Bcl‐2. These results indicated the protective effect of SBP on MG132‐induced apoptosis in RAW264.7 cells.