Open AccessEffects of storage on brown rice ( Oryza sativa L.) metabolites, analyzed using gas chromatography and mass spectrometry
Author(s) -
Wang Changyuan,
Feng Yuchao,
Zhang Shu,
Fu Tianxin,
Sheng Yanan,
Zhang Yiwei,
Jiang Yingjun,
Yu Miao,
Zhang Liyuan,
Zhang Dongjie
Publication year - 2020
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.1589
Subject(s) - metabolomics , oryza sativa , brown rice , chemistry , gas chromatography–mass spectrometry , metabolite , food science , gas chromatography , fatty acid , mass spectrometry , metabolic pathway , chromatography , metabolism , biochemistry , gene
Abstract Metabolomic studies were carried out using gas chromatography and mass spectrometry (GC‐MS) on Daohuaxiang variety rice ( Oryza sativa L.) from the Wuchang Geographical Indication Rice Protection Area in Heilongjiang Province, to investigate the effects of storage on brown rice metabolism. The data were subjected to principal component analysis (PCA), orthogonal partial least squares‐discriminant analysis (OPLS‐DA), and cluster analysis using software such as SIMCA. Analysis of the samples led to the identification of a total of 160 metabolites. No significant differences were found in the amount of metabolites before and after storage. A total of 31 differential metabolites were screened, and the changes in metabolite content showed a “reverse change” overall. Storage significantly changed the content of various metabolites in rice, with fatty acids impacted most significantly. Metabolic pathway analysis revealed that fatty acid biosynthesis is a key metabolic pathway in rice storage. The degradation of brown rice quality caused by storage is closely related to the composition and content of its metabolites, and that change in lipid content significantly affects brown rice quality during storage.