Open AccessIdentification and characterization of virulent Aeromonas hydrophila Ah17 from infected Channa striata in river Cauvery and in vitro evaluation of shrimp chitosan
Author(s) -
Samayanpaulraj Vignesh,
Sivaramapillai Muthukumar,
Palani Sankara Naynar,
Govindaraj Krishnaveni,
Velu Vijay,
Ramesh Uthandakalaipandian
Publication year - 2020
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.1416
Subject(s) - aeromonas hydrophila , biology , microbiology and biotechnology , shrimp , virulence , penaeus monodon , enterotoxin , aeromonas , bacteria , fishery , escherichia coli , biochemistry , gene , genetics
Abstract Aeromonas hydrophila , an inhabitant in the aquatic ecosystem is considered as an important foodborne bacterial zoonotic pathogen in aquaculture. The present study aimed to identify virulent A. hydrophila from naturally infected Channa striata in river Cauvery and in vitro evaluation of shrimp chitosan. Rimler Shotts (RS) and blood agar medium identified the presence of pathogenic Aeromonas sp. from the infected C. striata . A. hydrophila Ah17 was identified using 16S rRNA nucleotide sequence. Extracellular enzymes such as amylase, lipase, and protease were screened in A. hydrophila Ah17. Antibiotic susceptibility tests showed A. hydrophila Ah17 was highly resistant against β ‐lactam, glycopeptide, macrolides, phosphonic, fucidin, and oxazolidinone classes of antibiotics. Virulent genes such as hemolysin ( aer and hly) , heat‐labile enterotoxin ( act ), cytotonic heat‐stable enterotoxin ( ast ), elastase ( ahyB ), and lipase ( lip ) were identified. Growth and the viable cell population of virulent A. hydrophila Ah17 were significantly reduced in a dose‐dependent manner against shrimp chitosan (CHS) from Penaeus indicus ( P. indicus ). Thus, the present study isolated virulent A. hydrophila Ah17 from the environmental source and characterized in vitro with shrimp chitosan.