Open AccessEnhancement of glucose homeostasis through the PI3K/Akt signaling pathway by dietary with Agaricus blazei Murrill in STZ‐induced diabetic rats
Author(s) -
Wei Qi,
Li Jie,
Zhan Yishu,
Zhong Qiangui,
Xie Baogui,
Chen Lei,
Chen Bingzhi,
Jiang Yuji
Publication year - 2020
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.1397
Subject(s) - insulin receptor , medicine , endocrinology , pentose phosphate pathway , glucose homeostasis , citric acid cycle , protein kinase b , pyruvate kinase , kinase , glycolysis , glucose transporter , pi3k/akt/mtor pathway , biology , insulin , biochemistry , signal transduction , insulin resistance , metabolism
Agaricus blazei Murrill (ABM) is an edible fungus. This study investigated the protective role of ABM fruiting body against streptozotocin (STZ)‐induced diabetic rats. After 4 weeks of ABM supplement, glucose homeostasis was improved in diabetic rats. Quantitative real‐time and Western blot analyses suggested that ABM could promote the gene and protein expression level of insulin receptor, pyruvate dehydrogenase kinase, phospho‐kinase B, kinase B, phosphatidylinositol 3‐kinase, insulin receptor substrate 1, glucose transporter‐4, and glutamine synthetase, while inhibiting the expression of glycogen synthase kinase 3β and c‐jun N‐terminal kinase 1 and 2. According to multivariate and univariate statistical analysis, liver metabolite profiles of the normal and diabetic rats fed basal and experimental diet were clearly separated. The differential liver metabolites from diabetic rats fed basal and ABM diet‐related pathways including the glycolysis pathway, pentose phosphate pathway, tricarboxylic acid cycle, and oxidative phosphorylation were analyzed. A total of 18 potential biomarker metabolites were identified as differential biomarkers associated with ABM supplement diet.