Open AccessPolyphenols extract from lotus seedpod ( Nelumbo nucifera Gaertn.): Phenolic compositions, antioxidant, and antiproliferative activities
Author(s) -
Shen Yingbin,
Guan Yifu,
Song Xun,
He Jialiang,
Xie Zhenxing,
Zhang Youwei,
Zhang Hui,
Tang Dan
Publication year - 2019
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.1165
Subject(s) - nelumbo nucifera , lotus , polyphenol , antioxidant , traditional medicine , biology , chemistry , botany , food science , biochemistry , medicine
Seedpod, the nonedible portion of lotus ( Nelumbo nucifera Gaertn.), was reported to be rich in polyphenols. The objective of this study was to investigate the major bioactive polyphenols of the lotus seedpods. The total polyphenol content (TPC) from ethanol extract of lotus seedpod (PELS) was found to be 34.23 μg gallic acid equivalents (GAE)/mg extract. Four polyphenolic compounds were identified in the PELS, comprised of one flavan‐3‐ol (catechin) and three flavonoids (kaemferol, quercetin and hyperoside). In vitro antioxidant and antiproliferative properties of the PELS were evaluated. PELS exhibited 89.38%, 99.82%, 68.25%, and 95.82% scavenging activities against 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH), superoxide, hydroxyl, and 2,2ʹazinobis‐3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS) radicals, respectively, at 1.6 mg/ml. The Fe 3+ reducing power of PELS was 0.605 at 0.32 mg/ml, which is comparable to glutathione (GSH). The PELS showed 31.79% metal chelating capacity and 87.79% inhibition of linoleic acid auto‐oxidation at 1.6 mg/ml. PELS showed cytotoxicity toward HepG2 and LNcap cell lines in vitro with IC 50 values at 44.59 and 11.50 μg/ml, respectively. The findings of this study provide evidences that the inedible lotus seedpod could be a source for natural antioxidants and anticancer agents.