Open AccessSulfated glycosaminoglycans from crown‐of‐thorns Acanthaster planci – extraction and quantification analysis
Author(s) -
Bahrom Nur Afiqah,
Sirajudeen K. N. S.,
Yip George W.,
Latiff Aishah A.,
Ghazali Farid Che
Publication year - 2013
Publication title -
food science and nutrition
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.614
H-Index - 27
ISSN - 2048-7177
DOI - 10.1002/fsn3.10
Subject(s) - acanthaster , glycosaminoglycan , starfish , sulfation , sea cucumber , chemistry , biology , anatomy , biochemistry , fishery , ecology , great barrier reef , coral reef
Abstract In this article, the novel inventive steps for the extraction and quantification of sulfated glycosaminoglycan ( GAG ) from A canthaster planci starfish, generally known as crown‐of‐thorns ( COT ), are reported. Starfish have been implicated with collagenous distributions within their body anatomy, thus making it a prima facie fact searching for the possibility that GAG s can be isolated from COT . In this study, total‐, N ‐, and O ‐sulfated GAG s were extracted from three anatomical regions of the COT (integument, internal tissue, and coelomic fluid) and comparison was made. The result showed that body region of COT seemed to contain higher amount of sulfated GAG s as opposed to the arm region (55.79 ± 0.65 μ g/mg was the highest amount in the body extracted from its coelomic fluid and 32.28 ± 3.14 μ g/mg was the highest amount in the arm extracted from its internal tissue). COT 's integument and coelomic fluid from its body region possessed the highest total of sulfated GAG s content with no significant difference ( P < 0.05) between the two. All GAG s from COT comprised a higher percentage of N ‐sulfated GAG s than its counterpart, the O ‐sulfated GAG s. When compared with a similar previous study that used sea cucumbers as the sulfated GAG s source, COT possessed more total sulfated GAG s content per milligram as compared with the sea cucumber generally. This result seems to unveil this marine species' advantage per se pertaining to GAG s extraction biomass applicability. Thus, COT could now be the better alternative source for production technology of total‐, N ‐, and O ‐sulfated GAG s.