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Modelling an amperometric biosensor acting in a flowing liquid
Author(s) -
Ivanauskas F.,
Baronas R.
Publication year - 2008
Publication title -
international journal for numerical methods in fluids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.938
H-Index - 112
eISSN - 1097-0363
pISSN - 0271-2091
DOI - 10.1002/fld.1653
Subject(s) - biosensor , analyte , amperometry , diffusion , mechanics , flow (mathematics) , electrode , mass transfer , convection , chemistry , analytical chemistry (journal) , chromatography , materials science , physics , thermodynamics , nanotechnology , electrochemistry
This paper presents a mathematical model of the amperometric biosensor based on an electrode covered with an enzyme membrane. The model involves three regions: the enzyme layer where enzymatic reaction as well as mass transport by diffusion takes place, a diffusion‐limiting region where only diffusion takes place, and a convective region, where the analyte concentration is maintained constant. Using computer simulation the influence of the biosensor geometry as well as the flow intensity on the biosensor response was investigated. This paper also deals with the conditions when the mass transport in the exterior region may be neglected to simulate the biosensor response assuming that the buffer solution is in intense flow and in powerful motion. The digital simulation was carried out using the finite difference technique. Copyright © 2007 John Wiley & Sons, Ltd.