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Biological activities and chemical characterization of the Lebanese endemic plant Origanum ehrenbergii Boiss
Author(s) -
Dawra Michella,
El Rayess Youssef,
El Beyrouthy Marc,
Nehme Nancy,
El Hage Rima,
Taillandier Patricia,
Bouajila Jalloul
Publication year - 2021
Publication title -
flavour and fragrance journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.393
H-Index - 70
eISSN - 1099-1026
pISSN - 0882-5734
DOI - 10.1002/ffj.3646
Subject(s) - chemistry , dichloromethane , ethyl acetate , methanol , gallic acid , cyclohexane , traditional medicine , organic chemistry , food science , chromatography , antioxidant , solvent , medicine
Origanum ehrenbergii Boiss, endemic to Lebanon, is widely used in culinary preparations. The aim of this study is to assess the chemical composition and biological activities of four extracts obtained by consecutive extractions with four different solvents presenting an increasing polarity (cyclohexane, dichloromethane, ethyl acetate, and methanol). HPLC‐DAD analysis revealed nine compounds with 8 phenolics, while GC‐MS analysis allowed to detect 96 volatile compounds. Among the latter, some compounds were identified for the first time in plant extracts such as 2‐allyl‐phenyl tiglate (26); 2‐furylmethyl octanoate (28); 1H‐cycloprop[e]azulen‐7‐ol,decahydro‐1,1,7‐trimethyl‐4‐methylene‐,[1ar‐(1aα,4aα,7β,7aβ,7bα)] (30); 2‐(Z)‐propenylphenyl tiglate (32); 6‐hydroxy‐4,4,7a‐trimethyl‐5,6,7,7a‐tetrahydrobenzofuran‐2(4H)‐one (33); ent‐beyerane (37); and (E)‐2‐tridecenyl angelate (40). The methanol extract had the highest antioxidant activity with an IC 50 of 37.5 μg/mL. It also had the highest phenolic content with 207.2 mg of gallic acid equivalents (GAE)/g of dry weight. The inhibition percentage of the acetylcholinesterase by the methanol extract (56.9%), the ethyl acetate extract (58.6%), and the cyclohexane extract (60.0%) was almost the same at 50 µg/mL. Furthermore, the dichloromethane extract inhibited both the growth of the MCF‐7 cell line by 67.0% and HCT‐116 cell line by 35.7% at 50 μg/mL. The MIC of the dichloromethane extract against Listeria monocytogenes ATCC 19 115 and Listeria monocytogenes isolated from “fish filet” was 4 and 15 μg/mL, respectively, and that of the cyclohexane extract against Staphylococcus aureus was 19.5 μg/mL.