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Biological activity, and volatile and phenolic compounds from five Lamiaceae species
Author(s) -
Çelik Gonca,
Kılıç Gözde,
Kanbolat Şeyda,
Özlem Şener Sıla,
Karaköse Mustafa,
Yaylı Nurettin,
Karaoğlu Şengül Alpay
Publication year - 2021
Publication title -
flavour and fragrance journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.393
H-Index - 70
eISSN - 1099-1026
pISSN - 0882-5734
DOI - 10.1002/ffj.3636
Subject(s) - lamiaceae , chemistry , satureja , carvacrol , dpph , thymol , nepeta , trolox , antibacterial activity , sabinene , botany , traditional medicine , food science , essential oil , limonene , antioxidant , organic chemistry , bacteria , biology , medicine , genetics
Lamiaceae, a family of flowering plants, have been historically used as culinary herbs, shrubs and medicinal plants. With this study, the volatile organic compounds (VOCs) of the five species of the Lamiaceae family ( Thymus cilicicus , Clinopodium nepeta L., Nepeta italica L., Thymbra spicata L. and Ziziphora clinopodioides ) that grow in Turkey were identified by the SPME/GC‐FID/MS method. The five species were screened in order to reveal their antibacterial and antioxidant activities. Phenolic compound analyses of five species were performed by using RP‐HPLC with diode array detection. Among the total number of 73 compounds that were identified, α ‐pinene (2.2%‐13.8%), β ‐pinene (0.3%‐25.9%), p ‐cymene (0.7%‐17.9%), γ ‐terpinene (0.4%‐22.0%), carvacrol (0.1%‐40.2%), sabinene (1.2%‐34.2%), terpinen‐4‐ol (0.2%‐27.5%), (E) ‐ β ‐caryophyllene (0.1%‐13.8%) and (E) ‐ β ‐farnesene (0.4%‐19.7%) were found to be the most plentiful ones. The minimal inhibitory concentration (MIC) values of the methanol extracts of the five species of the Lamiaceae family were investigated, and the results were in the range of 23‐959 µg/mL; the most pronounced antibacterial activities were determined to be against Mycobacterium smegmatis (23‐291 µg/mL). All of the methanol extracts possessed effective antioxidant capacity (IC 50 : 1.82‐28.18 μmol/L Trolox/g sample) as estimated from the 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical‐scavenging assays. Additionally, the effect of phenolics, especially benzoic acid (86.27, 27.85, ‐, 13.11, 9.59 mg/g extract, respectively), on the biological properties of the methanol extracts of the five species of the Lamiaceae family, was investigated.