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Analytical contribution of deuterium 2D‐ NMR in oriented media to 2 H/ 1 H isotopic characterization: the case of vanillin
Author(s) -
Texier–Bonniot Tristan,
Berdagué Philippe,
Robins Richard J.,
Remaud Gérald,
Lesot Philippe
Publication year - 2018
Publication title -
flavour and fragrance journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.393
H-Index - 70
eISSN - 1099-1026
pISSN - 0882-5734
DOI - 10.1002/ffj.3441
Subject(s) - chemistry , isotopomers , vanillin , deuterium , isotopic labeling , deuterium nmr , nuclear magnetic resonance spectroscopy , nmr spectra database , carbon 13 nmr , stereochemistry , molecule , organic chemistry , spectral line , physics , quantum mechanics , astronomy
The evaluation of intramolecular 2 H or 13 C isotopic composition of vanillin by liquid‐state nuclear magnetic resonance ( NMR ) is an illustrative example of the analytical challenges associated with molecular authenticity/traceability investigations in food products, and the determination/understanding of metabolic/synthetic pathways. Although isotopic ratio monitoring by 13 C NMR (irm‐ 13 C NMR ) provides a valuable new source of isotopic information, the analysis of position‐specific ( 2 H/ 1 H) isotopic fractionation of vanillin remains incomplete because of the overlap of two aromatic 2 H resonances, and the impossibility of separating these fortuitous isochronous nuclei by isotropic NMR . Exploiting the analytical potential of 2 H 2D‐ NMR in media containing polypeptide‐based liquid crystals, we show that all aromatic monodeuterated isotopomers can be spectrally discriminated on the basis of 2 H residual quadrupolar couplings, Δν Q ( 2 H), thus enabling the determination of their relative proportions for the first time. After discussing the multiple relevant cofactors leading to the best discrimination and optimization of experimental conditions for reliable quantitative measurements by anisotropic 2D‐ NMR , a comparative analysis of six vanillin samples from diverse origins is reported. How the 2 H distribution might relate to the biosynthesis of vanillin is discussed.