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Opportunities for ultra‐high resolution analysis of essential oils using comprehensive two‐dimensional gas chromatography: a review
Author(s) -
Shellie Robert,
Marriott Philip
Publication year - 2003
Publication title -
flavour and fragrance journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.393
H-Index - 70
eISSN - 1099-1026
pISSN - 0882-5734
DOI - 10.1002/ffj.1225
Subject(s) - chemistry , chromatography , resolution (logic) , gas chromatography , plot (graphics) , two dimensional gas , essential oil , sample (material) , two dimensional chromatography , mass spectrometry , analytical chemistry (journal) , high resolution , sample preparation , quadrupole mass analyzer , column (typography) , biological system , artificial intelligence , statistics , computer science , mathematics , frame (networking) , telecommunications , remote sensing , biology , geology
In comprehensive 2D gas chromatography, the entire sample is simultaneously subjected to analysis on two capillary columns. By using a suitable modulation interface between the primary and secondary columns, hundreds of fast, second‐dimension chromatograms are produced. The data from these chromatograms are treated such that a 3D surface plot or a 2D contour plot of the components' individual retention times, on each column, as well as peak responses, are represented. In a properly tuned comprehensive 2D chromatogram, the individual sample components are spread throughout a 2D separation space, providing a signicant increase in the probability of resolving a greater number of sample components without increasing the analysis time. Comprehensive 2D–GC has proved useful for high‐resolution conventional essential oil analysis as well as high‐resolution enantioselective essential oil analysis. Combining comprehensive 2D–GC with either a quadrupole or time‐of‐ight mass spectrometer gives a powerful 3D analysis technique, which is extremely effective for complex sample analysis. The present status and opportunities arising from these ultra‐high resolution approaches are discussed herein. Copyright © 2003 John Wiley & Sons, Ltd.