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Development of an efficient GLC system with a mixed chiral stationary phase and its application to the separation of optical isomers
Author(s) -
Tamogami Shigeyuki,
Awano Kenichi,
Amaike Masayasu,
Takagi Yoshikazu,
Kitahara Takeshi
Publication year - 2001
Publication title -
flavour and fragrance journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.393
H-Index - 70
eISSN - 1099-1026
pISSN - 0882-5734
DOI - 10.1002/ffj.1009
Subject(s) - chemistry , enantiomer , cyclodextrin , monoterpene , chiral stationary phase , chiral column chromatography , phase (matter) , chromatography , organic chemistry
In order to overcome the limitations of the methodology for the separation of enantiomers in GLC analysis, we successfully developed a novel GLC column named ‘Chiramix’, coated with a mixture of two chiral stationary phases, heptakis‐(2,6‐di‐ O ‐methyl‐3‐ O ‐pentyl)‐β‐cyclodextrin (2,6‐Me‐3‐Pe‐β‐CD) and octakis‐(2,6‐di‐ O ‐methyl‐3‐ O ‐trifluoroacetyl)‐γ‐cyclodextrin (2,6‐Me‐3‐TFAc‐γ‐CD). It was proved that the ‘Chiramix’ column is greatly superior to those with a single chiral phase for separating enantiomers of compounds with various functional groups. As a successful example, this was applied to the analysis of multicomponent systems, such as peach flavours, and the enantiomeric purities of several components of monoterpene hydrocarbons, alcohols, ketones and lactones were determined exactly and simultaneously. Copyright © 2001 John Wiley & Sons, Ltd.