Open AccessTranscriptome and metabolome integration in sugarcane through culm development
Author(s) -
Perlo Virginie,
Furtado Agnelo,
Botha Frederik C.,
Margarido Gabriel R. A.,
HodgsonKratky Katrina,
Choudhary Hemant,
Gladden John,
Simmons Blake,
Henry Robert J.
Publication year - 2022
Publication title -
food and energy security
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.253
H-Index - 25
ISSN - 2048-3694
DOI - 10.1002/fes3.421
Subject(s) - metabolome , sucrose , biology , transcriptome , saccharum , sugar , food science , bagasse , biomass (ecology) , metabolic engineering , biochemistry , botany , microbiology and biotechnology , agronomy , gene , gene expression , metabolite
Sugarcane (Saccharum sp.) is a tropical and subtropical C4 plant with a high photosynthetic and carbon assimilation efficiency that stores sucrose. Culm biomass is also composed of bagasse fibre, a by‐product of the sugarcane industry. This high‐yielding grass, high in sucrose and lignocellulosic biomass, is considered an optimal feedstock as an alternative to fossil fuels and to produce a broad range of high‐value biomaterials. The ideal sugarcane production system would optimise the relative production of sugar and these new products. Multi‐omics correlation analysis was used to generate a global view of the essential metabolic pathways identifying critical genes involved in carbon partitioning during different stages of development. This research employed an unprecedented metabolic and transcriptomic dataset of 360 samples from a selection of 1440 culms of 24 genotypes at five different development stages. Chemical composition and metabolome analysis showed an increase through the culm development of lignin, sucrose, carbon, and amino acids such as aspartic acid, serine, alanine, methionine, threonine 3‐cyano‐L‐alanine, and citric acid. Transcriptome analysis revealed functionalities such as transcription, nucleotide transport and metabolism, and the biosynthesis of amino acids that are highly activated during the immature stage and highly down‐regulated during the most mature age.