Open AccessTaERF73 is associated with root depth, thousand‐grain weight and plant height in wheat over a range of environmental conditions
Author(s) -
Du Yan,
Li Chaonan,
Mao Xinguo,
Wang Jingyi,
Li Long,
Yang Jinwen,
Zhuang Mengjia,
Sun Daizhen,
Jing Ruilian
Publication year - 2022
Publication title -
food and energy security
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.253
H-Index - 25
ISSN - 2048-3694
DOI - 10.1002/fes3.325
Subject(s) - biology , cleaved amplified polymorphic sequence , haplotype , gene , single nucleotide polymorphism , coding region , molecular marker , agronomy , marker assisted selection , horticulture , genotype , genetics , restriction fragment length polymorphism
AP2/ERF is a plant‐specific transcription factor superfamily regulating plant growth, development and responses to multiple stresses. However, the roles of ERF genes in the growth and development of wheat ( Triticum aestivum L.) remain elusive. In this study, a novel ERF gene TaERF73 was cloned from wheat. qRT‐PCR analysis showed that TaERF73 is predominantly expressed in roots. It responds to treatments of auxin, MeJA, ABA, low temperature and drought stresses. Ectopic expression of TaERF73 in rice caused short root length, indicating that TaERF73 is a negative regulator of root growth. Four SNPs were detected in TaERF73 ‐ 4B coding region through sequence polymorphism analysis. A dCAPS (derived cleaved amplified polymorphic sequence) molecular marker was developed based on the SNP at 119 bp (G/C) of TaERF73 ‐ 4B . Association analysis between genotypes and agronomic traits revealed that Hap ‐4B‐2 is a haplotype of TaERF73 ‐ 4B associated with shorter plant height, higher thousand‐grain weight and longer root depth. The distributions of haplotypes in ten Chinese major wheat zones suggested that Hap ‐4B‐2 has been positively selected in the breeding process. Together, the ERF gene TaERF73 is associated with root depth, plant height and thousand‐grain weight under drought, heat and well‐watered conditions. The molecular marker developed in this study could be a valuable source for marker‐assisted selection and genetic improvement in wheat.