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Evaluation of the effect of reactive sulfide on the acute toxicity of silver (I) to Daphnia magna . Part 2: Toxicity results
Author(s) -
Bianchini Adalto,
Bowles Karl C.,
Brauner Colin J.,
Gorsuch Joseph W.,
Kramer James R.,
Wood Chris M.
Publication year - 2002
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620210626
Subject(s) - daphnia magna , toxicity , daphnia , acute toxicity , environmental chemistry , sulfide , chemical toxicity , cladocera , aquatic toxicology , toxicology , chemistry , biology , zoology , crustacean , organic chemistry
The protective effect of reactive sulfide against AgNO 3 toxicity to Daphnia magna neonates was studied. Acute (48‐h) toxicity tests were performed in the absence (<5 nM) and presence of low (∼25 nM) and high (∼250 nM) concentrations of zinc sulfide clusters under oxic conditions. In both the presence and the absence of sulfide, lower mean lethal concentration (LC50) values were observed when measured as opposed to nominal silver concentrations were used in calculations. This reflected the fact that measured total silver concentrations were lower than nominal concentrations due to losses of silver from solution observed during the experiment. High concentration (∼250 nM) of sulfide completely protected against toxicity up to the highest silver concentration tested (2 μg/L [19 nM]) with measured silver data. In the presence of environmentally realistic levels of sulfide (∼25 nM) in receiving waters, acute silver toxicity was reduced by about 5.5‐fold. However, when filtered (0.45 μm) silver concentrations alone were considered, toxicity (48‐h LC50) was similar in the absence (0.22 μg/L) and presence (0.28 μg/L) of sulfide. The difference between measured total and filtered silver was attributed to chemisorption of the metal sulfide onto the membrane filter and provides evidence that the toxic fraction of silver is that which is unbound to sulfide. Accumulation of silver was greater in daphnids exposed to silver in the presence of sulfide than in its absence, even though a toxic effect was not observed under these conditions. In this case, silver appears to be incorporated by daphnids rather than merely adsorbed on the surface. Our results point out the need to incorporate sulfide into the acute biotic ligand model and to assess its potentially large role in preventing chronic toxicity.