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Detection of environmental endocrine‐disruptor effects on gene expression in live Rana catesbeiana tadpoles using a tail fin biopsy technique
Author(s) -
Veldhoen Nik,
Helbing Caren C.
Publication year - 2001
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620201208
Subject(s) - rana , endocrine disruptor , biology , gene expression , endocrine system , fin , zoology , gene , endocrinology , hormone , genetics , materials science , composite material
We describe a novel method of rapidly assessing exposure of premetamorphic Rana catesbeiana tadpoles to endocrine‐disrupting chemicals. Tail fin biopsy and reverse transcription–polymerase chain reaction analyses of the thyroid hormone (TH)–responsive gene, TH receptor β (TRβ), demonstrate for the first time that a known accelerant of TH‐induced metamorphosis, acetochlor, can significantly enhance TH‐induced TRβ mRNA levels within 24 h at an environmentally relevant dose. Although we focus on laboratory exposures in this study, this method easily can be adapted for use in field studies.