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Dose‐ and time‐dependent formation of biliary benzo[ a ]pyrene metabolites in the marine flatfish DAB ( Limanda limanda )
Author(s) -
van Schanke Arne,
Holtz Frank,
van der Meer Jaap,
Boon Jan P.,
Ariese Freek,
Stroomberg Gerard,
van den Berg Martin,
Everaarts Jan M.
Publication year - 2001
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620200804
Subject(s) - chemistry , limanda , benzo(a)pyrene , pyrene , carcinogen , glutathione , environmental chemistry , cytochrome p450 , microsome , metabolism , enzyme , biochemistry , fish <actinopterygii> , flatfish , biology , fishery , organic chemistry
Polycyclic aromatic hydrocarbons (PAHs) are abundant pollutants, and many PAHs are carcinogenic, but only after metabolic activation. Benzo[ a ]pyrene (B a P) is among the most carcinogenic PAHs. The dose and time response of two enzymes involved in B a P metabolism and the amounts of B a P metabolites excreted into the bile were evaluated in an experiment with dab ( Limanda limandä ). Ninety dab were exposed orally to one of five doses of B a P (0, 0.08, 0.4, 2, or 10 mg/kg) and sampled at 3, 6, or 12 d after exposure. None of the doses studied caused significant induction of either microsomal ethoxyresorufin‐ O ‐deethylase (EROD), which reflects cytochrome P450 1A (CYP1A) activity, or cytosolic glutathione‐ S ‐transferase activity (GST). Concentrations of biliary B a P metabolites significantly increased with dose and significantly decreased with time after exposure. It is concluded that biliary B a P metabolites provide a much more sensitive method than EROD (CYP1A) or GST activity to monitor recent exposure to PAHs in dab.