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Effect of mercury and Gpi‐2 genotype on standard metabolic rate of eastern mosquitofish ( Gambusia holbrooki )
Author(s) -
Tatara, Christopher Paul,
Newman Michael C.,
Mulvey Margaret
Publication year - 2001
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620200413
Subject(s) - gambusia , mosquitofish , mercury (programming language) , biology , metabolic rate , zoology , poeciliidae , genotype , ecology , toxicology , genetics , fish <actinopterygii> , fishery , endocrinology , gene , computer science , programming language
Previous studies demonstrated differential mortality among mosquitofish of different Gpi‐2 genotypes during acute mercury and arsenate exposures. Mercury‐exposed mosquitofish also had Gpi‐2 genotype‐specific differences in glycolytic and Krebs cycle metabolite pools. The mortality and metabolite data suggested that mosquitofish bearing specific Gpi‐2 genotypes might differ in metabolic efficiency, with less efficient Gpi‐2 genotypes having higher standard metabolic rates (SMRs) and shorter times to death during acute mercury exposure. Effect of Gpi‐2 genotype on SMR was assessed with a factorial arrangement of six Gpi‐2 genotypes and two exposure sequences (Control — Control; Control — 100 μg/L Hg). The SMRs were estimated by measuring oxygen consumption using an indirect, closed‐circuit, computer‐controlled respirometer. A 48‐h exposure to 100 μg/L of mercury resulted in a 16.7% elevation of SMR above control levels ( p = 0.001). The Gpi‐2 genotype and the number of heterozygous loci per individual had no significant effect on SMR in mercury‐exposed mosquitofish. The experimental results do not support the hypothesis that Gpi‐2 genotype‐specific differences in glycolytic and Krebs cycle metabolite pools and mortality in mosquitofish exposed to mercury are associated with differences in SMR.