Premium
Separation and characterization of gall bladder bile metabolites from speckled trout, Salvelinus fontinalis , exposed to individual polycyclic aromatic compounds
Author(s) -
Leonard James D.,
Hellou Jocelyne
Publication year - 2001
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620200322
Subject(s) - salvelinus , fontinalis , trout , gall , toxicokinetics , zoology , biology , environmental chemistry , chemistry , fish <actinopterygii> , ecology , fishery , biochemistry , metabolism
Speckled trout, Salvelinus fontinalis , were orally exposed to individual polycyclic aromatic compounds (PACs) represented by benzo[a]pyrene, carbazole, chrysene, dibenzofuran, dibenzothiophene, fluorene, phenanthrene, and pyrene. Fish were sacrificed 7 d after exposure and the gall bladder removed for bile analysis. High pressure liquid chromatography (HPLC) with fluorescence (F) and ultraviolet (UV) detection was used to determine the presence of PAC derivatives in the bile without pretreatment. Glucuronide conjugates were predominant in all exposures with variable amounts (0–53%) of phenols and starting material. Identification of compounds was confirmed by selective extraction of less polar nonconjugated PACs and enzymatic hydrolysis of water‐soluble material. This was followed by HPLC and/or gas chromatography‐mass spectrometry (GCMS) characterization of the produced phenols. Total metabolite levels varied widely among compounds.