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Differential induction of glutathione S‐transferases in the clam ruditapes decussatus exposed to organic compounds
Author(s) -
Hoarau Pascal,
GnassiaBarelli Mauricette,
Romeo Michèe,
Girard JeanPierre
Publication year - 2001
Publication title -
environmental toxicology and chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.1
H-Index - 171
eISSN - 1552-8618
pISSN - 0730-7268
DOI - 10.1002/etc.5620200310
Subject(s) - glutathione , glutathione s transferase , isozyme , biochemistry , toxin , biology , western blot , chemistry , mycotoxin , microbiology and biotechnology , enzyme , food science , gene
Studies of glutathione S‐transferase (GST) induction were performed in the Mediterranean clam Ruditapes decussatus after controlled exposure to organics in holding tanks. Clams were treated with phenobarbital (PB), benzo[ a ]pyrene (B a P), and 2,2‐bis‐( p ‐chlorophenyl)‐1,1‐dichlorethylene ( p,p′ ‐DDE). Three different substrates, i.e., 1‐chloro‐2,4‐dinitrobenzene (CDNB), ethacrynic acid (ETHA), and paranitrobenzene chloride (PNBC), were used to determine GST activities in order to distinguish the isoenzymes induced by contamination. The isoforms conjugating ETHA were significantly induced by treatment with PB and BaP whereas exposure to p,p′ ‐DDE induced isoforms conjugating CDNB and ETHA. An antibody against affinity‐purified GSTs from R. decussatus was prepared by injection into rabbit. The serum containing the antibody gave a positive reaction with both the purified GSTs from R. decussatus and the low molecular weight GSTs from rat. Subcellular fractions from both control and treated animals were analyzed by Western blot. Cytosolic extracts from clams contaminated with PB and p,p′ ‐DDE showed a 24‐kDa band in addition to the 26‐kDa band recognized by the antibody. Results of these studies suggest that, in R. decussatus , organics may induce GSTs belonging to the π class.